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KMID : 1140120100150030204
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Cancer Prevention Research
2010 Volume.15 No. 3 p.204 ~ p.210
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Effects of Chitosan on the Production of TNF-α, IL-1β, and IL-6 in Mice
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Chung Yang-Sook
Choi Myung-Won
Park In-Dal
Park Kun-Young
Kim Kwang-Hyuk
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Abstract
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Chitosan is derived from chitin by a process of controlled deacetylation. In the present study, we investigated the effects of chitosan on the production of the cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in mice. The culture supernatants of splenocytes exposed with chitosan alone or chitosan plus cell stimulants such as lipopolysaccharide (LPS), concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) were harvested to assay TNF-α, IL-1β, and IL-10 production. TNF-α from splenocytes exposed with chitosan were increased compared to PBS control, but TNF-α in the culture supernatants from splenocytes exposed with LPS＋chitosan or Con A+chitosan for a short time (6 hrs) were lower than that of the groups exposed with LPS or Con A alone. The IL-1β and IL-6 production in the culture supernatants from splenocytes exposed with chitosan were similar to those in PBS control, but IL-1β and IL-6 production in the exposure with LPS+chitosan were higher than those of the groups exposed with LPS only. These findings demonstrate that chitosan upregulates the inflammation reaction by IL-1β and IL-6 and downregulates that by TNF-α in LPS-associated immunity.
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KEYWORD
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Chitosan, TNF-α, IL-1β, IL-6, Mice
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